Journal: The Journal of Cell Biology

Article Title: γ-Tubulin ring complexes regulate microtubule plus end dynamics

doi: 10.1083/jcb.200905060

Figure Lengend Snippet: γ-Tubulin localizes along interphase MTs. (A) Solubility property of γ-tubulin. Same legend as in , except Western blot analysis was performed using γ-tubulin antibodies. P, pellet; S, supernatant. (B–D) γ-TuRC components localize along interphase MTs. S2R + cells fixed in methanol (control; B) or permeabilized before formaldehyde fixation (control [C] or depleted cells [D]). Cells were stained using antibodies against γ-tubulin (rabbit R62: B [a], C [a], and D [a, c, and e]; or monoclonal GTU88: C [c]), against Dgrip84 (C, e), or against Dgp71WD (C, g). (right) MTs are shown in red, γ-TuRC components are shown in green, and chromosomes are shown in blue. (E) Live γ-tubulin–GFP S2 cells. A cell treated with taxol was examined live by recording GFP (a), and then fixed and stained for α-tubulin (b; n = 20). The arrowheads indicate MT bundles stained by γ-tubulin. Bars, 5 µm.

Article Snippet: Strains y 1 w 118 or w 118 were used as control strains, and mutant strains Dgrip75 175 , Dgrip84 R20 ( R20/FM7 ; ), and Dgp71WD GE30807 (GenExel-Sein, Inc.; ) were used for the study.

Techniques: Solubility, Western Blot, Control, Staining

Journal: British Journal of Clinical Pharmacology

Article Title: Plethysmography and impulse oscillometry assessment of tiotropium and ipratropium bromide; a randomized, double-blind, placebo-controlled, cross-over study in healthy subjects

doi: 10.1111/j.1365-2125.2006.02594.x

Figure Lengend Snippet: Baseline lung function data for 30 healthy subjects. Mean (SD) presented

Article Snippet: Pulmonary function measurements For IOS (Masterscreen IOS; Erich Jaeger, Hoechberg, Germany) subjects supported their cheeks to reduce upper airway shunting while impulses were applied during tidal breathing for 30 s. R5, R10, R15, R20 and R25 (respiratory resistance 5–25 Hz), X5 (reactance at 5 Hz) and RF (resonant frequency) were recorded.

Techniques:

Journal: British Journal of Clinical Pharmacology

Article Title: Plethysmography and impulse oscillometry assessment of tiotropium and ipratropium bromide; a randomized, double-blind, placebo-controlled, cross-over study in healthy subjects

doi: 10.1111/j.1365-2125.2006.02594.x

Figure Lengend Snippet: Effect of anticholinergic bronchodilators on IOS measurements in 30 healthy subjects. For IOS resistance measurements (R5 and R20), the mean difference compared to placebo is presented. R15 and R25 data (not shown) was similar to R5 and R20. RF data was log-transformed and the ratio of the geometric means compared to placebo is presented. X5 data (not shown) was similar to RF data. Error bars are 95% confidence intervals. •, Tio 54 mcg; ▪, Tio 18 mcg; ▴, IB 40 mcg

Article Snippet: Pulmonary function measurements For IOS (Masterscreen IOS; Erich Jaeger, Hoechberg, Germany) subjects supported their cheeks to reduce upper airway shunting while impulses were applied during tidal breathing for 30 s. R5, R10, R15, R20 and R25 (respiratory resistance 5–25 Hz), X5 (reactance at 5 Hz) and RF (resonant frequency) were recorded.

Techniques: Transformation Assay

Journal: British Journal of Clinical Pharmacology

Article Title: Plethysmography and impulse oscillometry assessment of tiotropium and ipratropium bromide; a randomized, double-blind, placebo-controlled, cross-over study in healthy subjects

doi: 10.1111/j.1365-2125.2006.02594.x

Figure Lengend Snippet: Weighted mean change (0–24 h) in lung function measurements compared to placebo

Article Snippet: Pulmonary function measurements For IOS (Masterscreen IOS; Erich Jaeger, Hoechberg, Germany) subjects supported their cheeks to reduce upper airway shunting while impulses were applied during tidal breathing for 30 s. R5, R10, R15, R20 and R25 (respiratory resistance 5–25 Hz), X5 (reactance at 5 Hz) and RF (resonant frequency) were recorded.

Techniques:

Journal: British Journal of Clinical Pharmacology

Article Title: Plethysmography and impulse oscillometry assessment of tiotropium and ipratropium bromide; a randomized, double-blind, placebo-controlled, cross-over study in healthy subjects

doi: 10.1111/j.1365-2125.2006.02594.x

Figure Lengend Snippet: Effect size calculations for treatments compared with placebo

Article Snippet: Pulmonary function measurements For IOS (Masterscreen IOS; Erich Jaeger, Hoechberg, Germany) subjects supported their cheeks to reduce upper airway shunting while impulses were applied during tidal breathing for 30 s. R5, R10, R15, R20 and R25 (respiratory resistance 5–25 Hz), X5 (reactance at 5 Hz) and RF (resonant frequency) were recorded.

Techniques:

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Growth Differentiation Factor‐15 Deficiency Inhibits Atherosclerosis Progression by Regulating Interleukin‐6–Dependent Inflammatory Response to Vascular Injury

doi: 10.1161/JAHA.112.002550

Figure Lengend Snippet: Oligonucleotides Used for Genotyping Polymerase Chain Reaction

Article Snippet: R20 reverse , 5′‐CCC AGT CTT GTA GAC AGA GCA A‐3′ , , Metabion, Planegg‐Martinsried, Germany.

Techniques: Sequencing, Amplification, Knock-Out